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TOPIC: The Periondental study by MOVE in Amsterdam

The Periondental study by MOVE in Amsterdam 12 Dec 2013 13:52 #1118

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As discussed during IFATS (which discussion was "eliminated by the site restore) - the highest interest I had in the presentation of Marco Helder- author of many many studies with SVF and who uses Celution for his studies- of the VU of Amsterdam and who basically studies everything related to bone. i.e orthopedics but also dentistry with his colleagues in house.

Looks great :happy:

A PHASE I CLINICAL TRIAL FOR MAXILLARY BONE AUGMENTATION WITH ADIPOSE STEM CELLS AND CALCIUM PHOSPHATE SCAFFOLDS; AN INTRA-OPERATIVE CONCEPT
Presenter: Marco N. Helder, PhD
Authors: Helder MN, Prins HJ, ten Bruggenkate CM,
Overman JR, Klein-Nulend J, Schulten EA
VU University Medical Center
Patients with require maxillary sinus floor elevation (MSFE) prior to dental implant placement. Synthetic bone substitutes are used as an alternative for the ‘gold standard’, i.e. autologous bone. However, bone substitutes only allow osteoconduction, since viable osteogenic cells are lacking. Cell-based bone tissue engineering is a promising technique to improve the bone forming capacity of bone ubstitutes. We evaluated the feasibility, safety and efficacy of combining a calcium phosphate as bone substitute with freshly isolated adipose
stem cells during a one-step surgical procedure for Maxillary Sinus Floor Elevation (MSFE). Osteoinductive implants (calcium phosphate (CaP) carriers seeded with the freshly isolated stromal vascular fraction of adipose tissue) were generated in an intra-operative procedure within the OR-complex within hours, thereby avoiding costly stem cell expansions and a second intervention. Where possible, a ‘split mouth design’ (with only CaP scaffold at the contralateral control side) was applied to allow efficacy evaluation. Adverse events (AE) were monitored, and clinical, X-ray, and Cone-beam CT data are collected at regular intervals during follow-up. After six months biopsies are obtained during dental implant placement, and evaluated for bone formation by histomorphometry and μCT.
Sofar, we included 10 patients. All patients uneventfully underwent an MSFE procedure and no adverse effects were reported during 1 year follow-up. Bone as well as osteoid percentage were significantly higher in bone biopsies taken from study sides than control sides throughout the complete biopsies, suggesting that bone formation does not only occur from the pre-existing sinus floor, and that the adipose stem cells may stimulate bone formation. This study demonstrated for the first time the feasibility, safety and potential efficacy of freshly isolated adipose stem cells with a calcium phosphate for MSFE, and provides the first step towards a novel treatment concept that might offer broad potential for cell-based regenerative medicine applications.

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The picture from the PDF was very poor quality but here it is anyway.

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Board moderator and Site-owner. I still regret the day I started analysing the prospects of MacroPore (now Cytori) back in 2004- a left-over from the tech-bubble at that time from the century change in my portfolio- and became addicted to Cytori´s fat cell technology. :cry:

The Periondental study by MOVE in Amsterdam 13 Dec 2013 08:25 #1120

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The range of applications for ADRC is truly fascinating. It truly has the ability to alter the treatment paradigm for every medical condition and will affect every practitioner/specialist in the not too distant future.

Here is another case which is fascinating and hopefully provides an element of confidence in CYTX ability to treat radiation induced injuries (BARDA here we come). The photos in the article (linked) are quite graphic and provide an impressive before/after view of the treatment.

www.egms.de/static/en/journals/gpras/2013-3/gpras000015.shtml

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The Periondental study by MOVE in Amsterdam 13 Dec 2013 08:55 #1121

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nice rongside...fistula, where is our partner ?

On your case study...Given the healing power of ADRC's and comparing figure 1 with 2... I wonder if they could have used a smaller graft. Of course second guess this particular case is futile imho....but perhaps a additional benefit in the future.

In figure 3...looks like adipose tissue was mixed in with the stem cell injection.

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The Periondental study by MOVE in Amsterdam 13 Dec 2013 17:20 #1127

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Here is another case which is fascinating and hopefully provides an element of confidence in CYTX ability to treat radiation induced injuries (BARDA here we come). The photos in the article (linked) are quite graphic and provide an impressive before/after view of the treatment.


On the predecessor site (Young-foxes.com 2009) there was already a similar study out of Hannover Germany. The evidence is overwhelming.

Still- as part of the third "portion" of the deal- BARDA requires reverse-modeling. (stupid really). Since efficacy already has been proven in the Akita clinics, it is required to show efficacy in animals. Primates- but than again in rodents - another US university brought out similar evidence (IFATS book -#82-

A

DIPOSE DERIVED STEM CELL THERAPY AMERLIORATES DELAYED WOUND HEALING AFTER RADIATION BY MODULATING TGF-BETA EXPRESSION
Presenter: Alex K. Wong, MD
Authors: Ragina N, Hoang D, Hill C, Lee YS, Kim G,
Han B, Hong Y, Chuong CM, Senagore A,
Urata MM, Wong AK
Keck School of Medicine of USC
Background: Recent studies have reported that Adipose Derived Stem Cells (ADSCs) in lipoaspirated fat could significantly improve non-healing, radiation-damaged wounds. However the mechanism of action is not completely understood, and limited animal studies have restricted approval of this technique in human patients. Therefore, we have developed a murine model of chronic radiation injury to etermine whether there is a beneficial effect of topical delivery of ADSCs on radiation-induced wound delay. Methods: To generate a murine model of chronic radiation- induced wound delay, an 8 x 4 cm field on the dorsal, lateral surface of Balb/c mice was irradiated 0Gy of radiation. After 6-8 weeks a 0.8cm full thickness circular wound was created utilizing the splinted wound model. ADSCs were derived from inguinal fat pads were from 6-7 week GFP transgenic mice using previously described protocols. Different quantities of ADSCs were applied topically to the wounds in a transglutaminase gel matrix. Fibro blast cells in the gel or gel alone without cells were used as controls.
Results: Wounds were assessed on post operative day 18 and 26. Topical delivery of 1x10^5 ADSCs to radiated wounds resulted in 2.5 and 2 times greater wound closure compared to 1x10^5 fibroblasts or no cells, respectively [p=.008 and p<.005]. By day 26 the rate of wound closure post ADSCs application, resembled that of the wound closure in unirradiated animals.
No difference in wound closure was observed in wounds that were not irradiated, with or without the application of ADSCs. Further analysis revealed that ADSC application reduced pathologic upregulated TGF-beta expression in
heal ingradiated wounds and decreased M2 Macrophage recruitment to the wound bed.
Conclusions: We have established a reproducible pre-clinical model of radiation-induced delayed wound healing in Balb/c mice. We demonstrated that purified ADSCs delivered topically in a biologic matrix significantly accelerated closure in wounds in chronically irradiated animals compared to the controls. This data can serve as a potential basis serve as a basis for IRB- approved clinical trial in patients with non-healing radiation induced wounds. Further studies are underway to better define the mechanism of action for this promising therapy.

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The Periondental study by MOVE in Amsterdam 13 Dec 2013 19:39 #1128

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***No difference in wound closure was observed in wounds that were not irradiated, with or without the application of ADSCs.***
That was interesting. I wonder why ?

Otherwise...to repeat a blogger I read...SHOW ME THE MONEY !!!!!!

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The Periondental study by MOVE in Amsterdam 15 Dec 2013 11:54 #1130

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***No difference in wound closure was observed in wounds that were not irradiated, with or without the application of ADSCs.***
That was interesting. I wonder why ?


Every mammalian has inherent healing capacity. According to Caplan- the most potent regenerative cells are MSC pre-cursors (i.e. undifferentiated MSCs- the so called pericytes)which are stored at the outer wall of all blood vessels.
As long as there is blood flow at the site of injury these endogenous cells will come to the rescue of the various stages of the healing process. It is where fibrous scarred tissue caused by circumstances like radiation have occurred that the injury requires outside help to get thru the stages of the healing process.

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Board moderator and Site-owner. I still regret the day I started analysing the prospects of MacroPore (now Cytori) back in 2004- a left-over from the tech-bubble at that time from the century change in my portfolio- and became addicted to Cytori´s fat cell technology. :cry:

The Periondental study by MOVE in Amsterdam 15 Dec 2013 18:23 #1131

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I understand there is a natural healing process present but for some reason I thought adding to the process would provide some aid in time of healing even if not compromised by lack of blood flow.

I guess not...thanks.

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