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TOPIC: Intellicell et al "clobbered" at IFATS in Quebec

Intellicell et al "clobbered" at IFATS in Quebec 31 Dec 2012 13:09 #26

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At IFATS- see picture
Cytori -after an absence for several years, possibly caused by "cool" personal relationships between some leading folks (Katz versus Hedrick as an example), three scientists Min Zhu, Kevin Hicok and John Fraser presented again at the 2012 meeting in Quebec- Canada and teared the "science" of newcomers in the field (IntelliCell, AdiStem etc etc) totally apart on a very scientific basis.

The details you can read in the following three posts....
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Board moderator and Site-owner. I still regret the day I started analysing the prospects of MacroPore (now Cytori) back in 2004- a left-over from the tech-bubble at that time from the century change in my portfolio- and became addicted to Cytori´s fat cell technology. :cry:

Intellicell et al "clobbered" at IFATS in Quebec 31 Dec 2012 13:52 #27

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Senior-Scientist Min Zhu from Cytori explained all there is to know about "ultrasonic cavitation" in order to extract ADRCs from fat tissue (IntelliCell) and the same for extraction with an emulsifier and centrifugation (AdiStem) :lol: :lol:

NON-ENZYMATIC METHODS TO OBTAIN REGENERATIVE CELLS FROM ADIPOSE: IS IT PRACTICAL OR EVEN POSSIBLE?
Presenter: Min Zhu, MD
Authors: Zhu M, Hicok KC, Shanahan R, Yu J, Souverneva O, Fornace G, Alfonso Z, Arm D, Fraser JK Cytori Therapeutics Inc
Recently, non-enzymatic methods to obtain stromal vascular cells (SVF) from fat have been proposed. We report our findings for the evaluation of these two methods; one using an emulsifier and the other utilizing ultrasonic energy.
Methods: Intra-donor sample comparison studies were performed. Human adipose was processed using the gold standard enzymatic process or using either a commercially available emulsifier-based cell isolation kit (Adistem®) or exposure to ultrasonic energy varying in power and amplitude. The resultant cells obtained by the different methods were assessed for cell yield and viability, the frequency of CFU-F, and cellular composition based on CD marker protein expression.
Results: Compared to the enzymatic approach, the emulsifier based method yielded primarily red blood cells and 10 times less nucleated cells than the enzymatic method. The majority of these nucleated cells were blood-derived granulocytes. No adherent colonies were detected in the CFU-F assay, whereas the CFU-F frequency of enzyme-derived SVF ranged from 0.5-2% of plated cells. High energy ultrasound destroyed fat and yielded mainly cellular debris. The viable nucleated cell numbers obtained with control (zero-power), low, and medium acoustic energy settings were only 13.7 ± 4.9%, 13.0 ± 6.3% and 11.2 ± 6.5% of that obtained enzymatically (n=5, p<0.02). Nucleated cells in ultrasonically treated samples were primarily white blood cells, regardless of power setting. CD34+ cell content was between 14.5 to 22.2 times less within ultrasound samples and exhibited a CFU-F frequency that was 6- to 15-fold less than enzyme-derived samples (p<0.05) for low and medium energy, respectively.
Conclusions: These results clearly demonstrate that an enzyme-based method is required to obtain the classically
defined stromal vascular fraction from adipose tissue. Neither of the alternative methods yielded comparable
populations in terms of cell number, identity, or viability. Until claims of alternative method efficacy are supported by rigorous scientific data, enzymatic isolation is and will remain the gold standard approach to obtain SVF.

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Board moderator and Site-owner. I still regret the day I started analysing the prospects of MacroPore (now Cytori) back in 2004- a left-over from the tech-bubble at that time from the century change in my portfolio- and became addicted to Cytori´s fat cell technology. :cry:

Intellicell et al "clobbered" at IFATS in Quebec 31 Dec 2012 13:58 #28

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B) Representation of the "ultrasound" scam decided not to present at the meeting after this "clobbering"

39
VIABILITY OF ISOLATED ADIPOSE-DERIVED STEM CELLS VIA ULTRASONIC SEPARATION
Presenter: Joseph A. Broujerdi, MD, DMD
Authors: Broujerdi JA, Schendel SA, Jacobson RL
DID NOT PRESENT AT THE MEETING

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Board moderator and Site-owner. I still regret the day I started analysing the prospects of MacroPore (now Cytori) back in 2004- a left-over from the tech-bubble at that time from the century change in my portfolio- and became addicted to Cytori´s fat cell technology. :cry:

Intellicell et al "clobbered" at IFATS in Quebec 31 Dec 2012 14:45 #29

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Fas I had my cells Lipoed and stored at a new facility in Toronto Canada by Doctor Sammy Sliwin. (Great doctor by the way)
This is the first adipose stem cell bank in Canada
Unfortunatley they use Adistem.... Oh well...

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Intellicell et al "clobbered" at IFATS in Quebec 31 Dec 2012 15:27 #30

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Sorry to hear that Joey.

Hopefully the year of Cytori brings you reasonable fast compensation for this anticipated write-off.
At least I wouldn't use the stored cells for any future treatment, if I were you.

In general- my sincere belief is that our belly is the best storage medium for ADRCs period and storage you do only when for some reason you have a liposuction. Argumentation- since I believe its not a very pleasant procedure, you avoid it in case of need the next time around.

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Board moderator and Site-owner. I still regret the day I started analysing the prospects of MacroPore (now Cytori) back in 2004- a left-over from the tech-bubble at that time from the century change in my portfolio- and became addicted to Cytori´s fat cell technology. :cry:
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